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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Angiopoietin 1 Monoclonal antibody, PBS Only

Angiopoietin 1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C4A3

Cat no : 68618-1-PBS

Synonyms

ANGPT1, ANG-1, ANG1, ANG 1, AGPT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Angiopoietin 1 Monoclonal antibody (68618-1-Ig)

    WB analysis using 68618-1-Ig (same clone as 68618-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68618-1-Ig (ANGPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68618-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Angiopoietin 1 Monoclonal antibody (68618-1-Ig)

    IF Staining of HUVEC using 68618-1-Ig (same clone as 68618-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Angiopoietin 1 antibody (68618-1-Ig, Clone: 3C4A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68618-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68618-1-PBS targets Angiopoietin 1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Angiopoietin 1 fusion protein Ag18829 相同性解析による交差性が予測される生物種
Full Name angiopoietin 1
Calculated molecular weight 498 aa, 58 kDa
Observed molecular weight70 kDa, 55 kDa
GenBank accession numberBC152411
Gene symbol Angiopoietin 1
Gene ID (NCBI) 284
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.