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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Angiopoietin-like 3/ANGPTL3 Recombinant antibody (83165-3-RR)

    WB analysis using 83165-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83165-3-RR (Angiopoietin-like 3/ANGPTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using Angiopoietin-like 3/ANGPTL3 Recombinant antibody (83165-3-RR)

    WB analysis of A375 using 83165-3-RR

    A375 cells were subjected to SDS PAGE followed by western blot with 83165-3-RR (Angiopoietin-like 3/ANGPTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Angiopoietin-like 3/ANGPTL3 Recombinant antibody

Angiopoietin-like 3/ANGPTL3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83165-3-RR
Clone No.230498F11

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

angiopoietin like 3, Angptl3, hypl

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, A375 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83165-3-RR targets Angiopoietin-like 3/ANGPTL3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name angiopoietin-like 3
Calculated molecular weight53KD
Observed molecular weight 54 kDa, 70 kDa
GenBank accession numberNM_013913.4
Gene Symbol Angptl3
Gene ID (NCBI) 30924
RRIDAB_3670860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9R182
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANGPTL3 belongs to the angiopoietin-like protein family. ANGPTL3 is mainly synthesized by liver cells and is notably expressed in kidney podocytes. Accumulating evidences have revealed that ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis and haematopoietic function and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. Thus, ANGPTL3 may serve as a potential biomarker in these diseases. ANGPTL3 belongs to a multifunctional secreted protein that mainly expresses in the liver, and is regulated by numerous post-translational modifications, including multiple cleavage and glycosylation (PMID: 30615486).

Protocols

Product Specific Protocols
WB protocol for Angiopoietin-like 3/ANGPTL3 antibody 83165-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols