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  • Featured Product
  • KD/KO Validated

Annexin A1 Polyclonal antibody, PBS Only

Annexin A1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55018-1-PBS

Synonyms

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26

Validation Data Gallery

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  • Western Blot (WB) analysis of C2C12 cells using Annexin A1 Polyclonal antibody (55018-1-AP)

    WB analysis of C2C12 using 55018-1-AP (same clone as 55018-1-PBS)

    C2C12 cell were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using Annexin A1 Polyclonal antibody (55018-1-AP)

    WB analysis of C6 using 55018-1-AP (same clone as 55018-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Annexin A1 Polyclonal antibody (55018-1-AP)

    WB analysis of HeLa using 55018-1-AP (same clone as 55018-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Annexin A1 Polyclonal antibody (55018-1-AP)

    WB analysis of HepG2 using 55018-1-AP (same clone as 55018-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using Annexin A1 Polyclonal antibody (55018-1-AP)

    WB analysis of K-562 using 55018-1-AP (same clone as 55018-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Annexin A1 Polyclonal antibody (55018-1-AP)

    WB analysis of NIH/3T3 using 55018-1-AP (same clone as 55018-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin A1 Polyclonal antibody (55018-1-AP)

    IHC staining of human colon using 55018-1-AP (same clone as 55018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 55018-1-AP (Annexin A1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Annexin A1 Polyclonal antibody (55018-1-AP)

    IHC staining of human oesophagus using 55018-1-AP (same clone as 55018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using Annexin A1 Polyclonal antibody (55018-1-AP)

    IHC staining of human oesophagus using 55018-1-AP (same clone as 55018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin A1 Polyclonal antibody (55018-1-AP)

    IHC staining of human colon using 55018-1-AP (same clone as 55018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin A1 Polyclonal antibody (55018-1-AP)

    IHC staining of human colon using 55018-1-AP (same clone as 55018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55018-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55018-1-PBS targets Annexin A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name annexin A1
Calculated molecular weight 40 kDa
Observed molecular weight 39 kDa
GenBank accession numberNM_000700
Gene symbol Annexin A1
Gene ID (NCBI) 301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes. The antibody is specific to ANXA1.