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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Annexin A2 Monoclonal antibody

Annexin A2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C1E12

Cat no : CL488-66035

Synonyms

Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using CoraLite® Plus 488-conjugated Annexin A2 Monoclona (CL488-66035)

    IF Staining of T-47D using CL488-66035

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using CoraLite® Plus 488-conjugated Annexin A2 antibody (CL488-66035, Clone: 1C1E12 ) at dilution of 1:600.

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Tested Applications

Positive IF/ICC detected inT-47D cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66035 is Annexin A2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Annexin A2 fusion protein Ag16654 相同性解析による交差性が予測される生物種
Full Name annexin A2
Calculated molecular weight 339 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC016774
Gene symbol Annexin A2
Gene ID (NCBI) 302
RRIDAB_2919279
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Annexin A2 antibody CL488-66035Download protocol
Standard Protocols
Click here to view our Standard Protocols