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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis using 66405-1-Ig (same clone as 66405-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis of Caco-2 using 66405-1-Ig (same clone as 66405-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis using 66405-1-Ig (same clone as 66405-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis of L02 using 66405-1-Ig (same clone as 66405-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Annexin A3 Monoclonal antibody (66405-1-Ig)

    WB analysis of HEK-293 using 66405-1-Ig (same clone as 66405-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66405-1-Ig (Annexin A3 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Annexin A3 Monoclonal antibody (66405-1-Ig)

    IHC staining of human pancreas using 66405-1-Ig (same clone as 66405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Annexin A3 Monoclonal antibody (66405-1-Ig)

    IHC staining of human pancreas using 66405-1-Ig (same clone as 66405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66405-1-Ig (Annexin A3 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66405-1-PBS in a different storage buffer formulation.

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Annexin A3 Monoclonal antibody, PBS Only

Annexin A3 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66405-1-PBS
Clone No.1A11E4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ANXA3, 1A11E4, 35 alpha calcimedin, 35-alpha calcimedin, Annexin 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66405-1-PBS targets Annexin A3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Annexin A3 fusion protein Ag2381 相同性解析による交差性が予測される生物種
Full Name annexin A3
Calculated molecular weight 322 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000871
Gene Symbol Annexin A3
Gene ID (NCBI) 306
RRIDAB_2881779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12429
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.