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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Annexin A4 Polyclonal antibody, PBS Only

Annexin A4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10087-1-PBS

Synonyms

ANXA4, ANX4, Annexin-4, Annexin IV, Annexin 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A4 Polyclonal antibody (10087-1-AP)

    WB analysis using 10087-1-AP (same clone as 10087-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 10087-1-AP (Annexin IV antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10087-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using Annexin A4 Polyclonal antibody (10087-1-AP)

    WB analysis of RAW 264.7 using 10087-1-AP (same clone as 10087-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10087-1-AP (Annexin IV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10087-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin A4 Polyclonal antibody (10087-1-AP)

    IHC staining of human colon using 10087-1-AP (same clone as 10087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10087-1-AP (Annexin A4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10087-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10087-1-PBS targets Annexin A4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A4 fusion protein Ag0130 相同性解析による交差性が予測される生物種
Full Name annexin A4
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000182
Gene symbol Annexin A4
Gene ID (NCBI) 307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.