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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Annexin A4 Monoclonal antibody, PBS Only

Annexin A4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10B5

Cat no : 60288-2-PBS

Synonyms

35-beta calcimedin, Annexin A4, Annexin IV, Annexin-4, ANXA4, Chromobindin-4, Endonexin I, Lipocortin IV

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using Annexin A4 Monoclonal antibody (60288-2-Ig)

    WB analysis of COLO 320 using 60288-2-Ig (same clone as 60288-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Annexin A4 Monoclonal antibody (60288-2-Ig)

    WB analysis of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Annexin A4 Monoclonal antibody (60288-2-Ig)

    WB analysis of A549 using 60288-2-Ig (same clone as 60288-2-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Annexin A4 Monoclonal antibody (60288-2-Ig)

    WB analysis of human heart using 60288-2-Ig (same clone as 60288-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60288-2-Ig (Annexin IV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Annexin A4 Monoclonal antibody (60288-2-Ig)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Annexin A4 Monoclonal antibody (60288-2-Ig)

    IHC staining of human placenta using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60288-2-Ig (Annexin IV antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin A4 Monoclonal antibody (60288-2-Ig)

    IF Staining of HeLa using 60288-2-Ig (same clone as 60288-2-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60288-2-Ig(Annexin IV antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60288-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60288-2-PBS targets Annexin A4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen Annexin A4 fusion protein Ag0130 相同性解析による交差性が予測される生物種
Full Name annexin A4
Calculated molecular weight 36 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC000182
Gene symbol Annexin A4
Gene ID (NCBI) 307
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Annexin A4 (Anxa4) is one of the Ca2 +-regulated and phospholipid-binding annexin superfamily proteins, and is located at 2p13. Anxa4 has a potential role in diagnosis, prognosis, and treatment of certain cancers. Annexin A4 shares 45 to 59% identity with other members and possibly interacts with ATP. Annexin A4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. The Annexin A4 protein is expressed in a wide range of tissues, associated with polarized epithelial cells and is proposed to be involved in the regulation of chloride ions across the epithelial membrane.