Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)
Various cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)
WB result of Annexin V antibody (66245-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin V transfected HeLa cells. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66245-1-Ig (same clone as 66245-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 66245-1-Ig (same clone as 66245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 66245-1-Ig (same clone as 66245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66245-1-Ig (same clone as 66245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66245-1-Ig (same clone as 66245-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (66245-1-Ig, Clone: 1E6A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
FC experiment of NIH/3T3 using 66245-1-Ig (same clone as 66245-1-PBS)
1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Annexin V Monoclonal antibody (66245-1-Ig, Clone:1E6A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
×
1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Annexin V Monoclonal antibody (66245-1-Ig, Clone:1E6A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin V Monoclonal antibody (66245-1-Ig, Clone:1E6A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin V Monoclonal antibody (66245-1-Ig, Clone:1E6A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Annexin V Monoclonal antibody, PBS Only

Annexin V Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 66245-1-PBS

Clone No.1E6A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Annexin A5, ANXA5, ANX5, Annexin-5, Annexin 5

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66245-1-PBS targets Annexin V in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Annexin V fusion protein Ag1538 相同性解析による交差性が予測される生物種
Full Name	annexin A5
Calculated molecular weight	36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC001429
Gene Symbol	Annexin V
Gene ID (NCBI)	308
RRID	AB_2881634
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P08758
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

WB analysis of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

WB analysis of HEK-293 using 66245-1-Ig (same clone as 66245-1-PBS)

IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

IHC staining of human spleen using 66245-1-Ig (same clone as 66245-1-PBS)

IHC staining of human spleen using 66245-1-Ig (same clone as 66245-1-PBS)

IHC staining of human colon using 66245-1-Ig (same clone as 66245-1-PBS)