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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Annexin VI Monoclonal antibody

Annexin VI Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2H7G7

Cat no : 68086-1-Ig

Synonyms

Annexin A6, ANXA6, ANX6, Annexin-6, Annexin 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin VI Monoclonal antibody (68086-1-Ig)

    WB analysis using 68086-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68086-1-Ig (Annexin VI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using Annexin VI Monoclonal antibody (68086-1-Ig)

    WB analysis using 68086-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68086-1-Ig (ANXA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Annexin VI Monoclonal antibody (68086-1-Ig)

    IF Staining of HepG2 using 68086-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Annexin VI antibody (68086-1-Ig, Clone: 2H7G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of K-562 cells using Annexin VI Monoclonal antibody (68086-1-Ig)

    FC experiment of K-562 using 68086-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Annexin VI (68086-1-Ig, Clone:2H7G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, human placenta tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68086-1-Ig targets Annexin VI in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Annexin VI fusion protein Ag3263 相同性解析による交差性が予測される生物種
Full Name annexin A6
Calculated molecular weight 673 aa, 76 kDa
Observed molecular weight67 kDa
GenBank accession numberBC017046
Gene symbol Annexin VI
Gene ID (NCBI) 309
RRIDAB_2918823
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

Protocols

Product Specific Protocols
WB protocol for Annexin VI antibody 68086-1-IgDownload protocol
IF protocol for Annexin VI antibody 68086-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols