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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Apolipoprotein A II/APOA2 Monoclonal antibody (66773-1-Ig)

    WB analysis of human plasma using 66773-1-Ig (same clone as 66773-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66773-1-Ig (Apolipoprotein A II/APOA2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Apolipoprotein A II/APOA2 Monoclonal antibody (66773-1-Ig)

    IHC staining of human liver cancer using 66773-1-Ig (same clone as 66773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66773-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Apolipoprotein A II/APOA2 Monoclonal antibody (66773-1-Ig)

    IHC staining of human liver cancer using 66773-1-Ig (same clone as 66773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66773-1-Ig (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66773-1-PBS in a different storage buffer formulation.

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Apolipoprotein A II/APOA2 Monoclonal antibody, PBS Only

Apolipoprotein A II/APOA2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66773-1-PBS
Clone No.1G1A2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66773-1-PBS targets Apolipoprotein A II/APOA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Apolipoprotein A II/APOA2 fusion protein Ag9863 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-II
Calculated molecular weight 100 aa, 11 kDa
Observed molecular weight 8-11 kDa
GenBank accession numberBC005282
Gene Symbol APOA2
Gene ID (NCBI) 336
RRIDAB_2882119
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02652
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)