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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Arginase-1 Monoclonal antibody (66129-1-Ig)

    WB analysis using 66129-1-Ig (same clone as 66129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66129-1-Ig (Arginase-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using Arginase-1 Monoclonal antibody (66129-1-Ig)

    IP experiment of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    IP result of anti-Arginase-1 (IP:66129-1-Ig, 4ug; Detection:66129-1-Ig 1:10000) with mouse liver tissue lysate 2240 ug. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Arginase-1 Monoclonal antibody (66129-1-Ig)

    IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Arginase-1 Monoclonal antibody (66129-1-Ig)

    IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Arginase-1 Monoclonal antibody (66129-1-Ig)

    IHC staining of human liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Arginase-1 Monoclonal antibody (66129-1-Ig)

    IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Arginase-1 Monoclonal antibody (66129-1-Ig)

    IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Arginase-1 Monoclonal antibody (66129-1-Ig)

    IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Arginase-1 Monoclonal antibody (66129-1-Ig)

    IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Arginase-1 Monoclonal antibody (66129-1-Ig)

    IF Staining of HepG2 using 66129-1-Ig (same clone as 66129-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Arginase-1 Monoclonal antibody, PBS Only

Arginase-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 66129-1-PBS
Clone No.5D6D12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

ARG1, Arginase 1, liver Arginase, arginase, liver, Arg-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66129-1-PBS targets Arginase-1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Arginase-1 fusion protein Ag8810 相同性解析による交差性が予測される生物種
Full Name arginase, liver
Calculated molecular weight236aa,25 kDa; 322aa,35 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC005321
Gene Symbol Arginase-1
Gene ID (NCBI) 383
RRIDAB_2881528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05089
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).