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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Arginase-1 Recombinant antibody, PBS Only

Arginase-1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4M7

Cat no : 82975-1-PBS

Synonyms

ARG1, Arginase 1, arginase, liver, Liver type arginase, Type I arginase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Arginase-1 Recombinant antibody (82975-1-RR)

    WB analysis using 82975-1-RR (same clone as 82975-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82975-1-RR (Arginase-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Arginase-1 Recombinant antibody (82975-1-RR)

    IHC staining of mouse liver using 82975-1-RR (same clone as 82975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82975-1-RR (Arginase-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Arginase-1 Recombinant antibody (82975-1-RR)

    IHC staining of mouse liver using 82975-1-RR (same clone as 82975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82975-1-RR (Arginase-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Arginase-1 Recombinant antibody (82975-1-RR)

    IF Staining of mouse liver using 82975-1-RR (same clone as 82975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Arginase-1 antibody (82975-1-RR, Clone: 4M7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82975-1-PBS

    Affinity and Kinetic Characterization of 82975-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82975-1-RR against Human Arginase-1 were performed. The affinity constant is 4.77 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82975-1-PBS targets Arginase-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Arginase-1 fusion protein Ag8595 相同性解析による交差性が予測される生物種
Full Name arginase, liver
Calculated molecular weight236aa,25 kDa; 322aa,35 kDa
Observed molecular weight 35-36 kDa
GenBank accession numberBC005321
Gene symbol Arginase-1
Gene ID (NCBI) 383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). ARG1 mainly expresses in neurons in a normal brain. The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).