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Arp5/ACTR5 Monoclonal antibody

Arp5/ACTR5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E11C1

Cat no : 67326-1-Ig

Synonyms

Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, FLJ12785, hARP5, INO80M, Sarcoma antigen NY SAR 16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Arp5/ACTR5 Monoclonal antibody (67326-1-Ig)

    WB analysis using 67326-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Arp5/ACTR5 Monoclonal antibody (67326-1-Ig)

    WB analysis of HEK-293 using 67326-1-Ig

    WB result of Arp5/ACTR5 antibody (67326-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells.

  • Western Blot (WB) analysis of C2C12 cells using Arp5/ACTR5 Monoclonal antibody (67326-1-Ig)

    WB analysis of C2C12 using 67326-1-Ig

    C2C12 cells were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig skeletal muscle tissue using Arp5/ACTR5 Monoclonal antibody (67326-1-Ig)

    WB analysis of pig skeletal muscle using 67326-1-Ig

    pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using Arp5/ACTR5 Monoclonal antibody (67326-1-Ig)

    WB analysis of rat skeletal muscle using 67326-1-Ig

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, C2C12 cells, pig skeletal muscle tissue, rat skeletal muscle tissue, HEK-293 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67326-1-Ig targets Arp5/ACTR5 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Arp5/ACTR5 fusion protein Ag15976 相同性解析による交差性が予測される生物種
Full Name ARP5 actin-related protein 5 homolog (yeast)
Calculated molecular weight 607 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC038402
Gene symbol ARP5/ACTR5
Gene ID (NCBI) 79913
RRIDAB_2882585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)

Protocols

Product Specific Protocols
WB protocol for Arp5/ACTR5 antibody 67326-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols