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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    WB analysis using 68316-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    IHC staining of rat brain using 68316-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    IHC staining of rat brain using 68316-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    IF Staining of rat brain using 68316-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    IF Staining of rat brain using 68316-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Ataxin 2 Monoclonal antibody

Ataxin 2 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68316-1-Ig
Clone No.1E8A2

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

ataxin 2, ATX2, ATXN2, FLJ46772, SCA2, TNRC13

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68316-1-Ig targets Ataxin 2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Ataxin 2 fusion protein Ag17402 相同性解析による交差性が予測される生物種
Full Name ataxin 2
Calculated molecular weight 1313 aa, 140 kDa
Observed molecular weight140-150 kDa
GenBank accession numberBC114546
Gene Symbol ATXN2
Gene ID (NCBI) 6311
RRIDAB_3085052
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDQ99700
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]

Protocols

Product Specific Protocols
WB protocol for Ataxin 2 antibody 68316-1-IgDownload protocol
IHC protocol for Ataxin 2 antibody 68316-1-IgDownload protocol
IF protocol for Ataxin 2 antibody 68316-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols