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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ataxin 2 Monoclonal antibody

Ataxin 2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E8A2

Cat no : 68316-1-Ig

Synonyms

ataxin 2, ATX2, ATXN2, FLJ46772, SCA2, TNRC13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    WB analysis using 68316-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    IHC staining of rat brain using 68316-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    IHC staining of rat brain using 68316-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68316-1-Ig (Ataxin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    IF Staining of rat brain using 68316-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Ataxin 2 Monoclonal antibody (68316-1-Ig)

    IF Staining of rat brain using 68316-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Ataxin 2 antibody (68316-1-Ig, Clone: 1E8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68316-1-Ig targets Ataxin 2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Ataxin 2 fusion protein Ag17402 相同性解析による交差性が予測される生物種
Full Name ataxin 2
Calculated molecular weight 1313 aa, 140 kDa
Observed molecular weight140-150 kDa
GenBank accession numberBC114546
Gene symbol ATXN2
Gene ID (NCBI) 6311
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATXN2 contains a repeat structure with 22 or 23 triplets coding for glutamine and the (CAG)8CAA(CAG)4CAA(CAG)8 sequence; expansion of this domain to a size ≥34 triplets with a pure CAG sequence primarily causes autosomal dominant SCA2 [PMID:18418684], while ATXN2 expansions with CAA interruptions were observed as the cause of Levo-dopa responsive Parkinson's disease [PMID:10668726]. ATXN2 expansions associated with ALS were reported to be interrupted by at least one CAA triplet [PMID:21537950]

Protocols

Product Specific Protocols
WB protocol for Ataxin 2 antibody 68316-1-IgDownload protocol
IHC protocol for Ataxin 2 antibody 68316-1-IgDownload protocol
IF protocol for Ataxin 2 antibody 68316-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Aditya (Verified Customer) (10-08-2024)

sensitive enough to use flourescent secondary. fantastic

  • Applications: Western Blot