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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse Atox1 Recombinant antibody, PBS Only (Capture)

Atox1 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241566A7

Cat no : 84305-1-PBS

Synonyms

HAH1, Atx1, 241566A7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Atox1 Recombinant antibody (84305-1-RR)

    WB analysis using 84305-1-RR (same clone as 84305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84305-1-RR (Atox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Atox1 Recombinant antibody (84305-1-RR)

    IHC staining of mouse heart using 84305-1-RR (same clone as 84305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84305-1-RR (Atox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84305-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01193-1

    Cytometric bead array standard curve of MP01193-1

    Cytometric bead array standard curve of MP01193-1, MOUSE Atox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84305-1-PBS. Detection antibody: 84305-2-PBS. Standard: Ag35290. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84305-1-PBS targets Atox1 as part of a matched antibody pair:

MP01193-1: 84305-1-PBS capture and 84305-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Atox1 fusion protein Ag35290 相同性解析による交差性が予測される生物種
Full Name ATX1 (antioxidant protein 1) homolog 1 (yeast)
Calculated molecular weight7 kDa
Observed molecular weight7 kDa
GenBank accession numberNM_009720
Gene symbol Atox1
Gene ID (NCBI) 11927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.