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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Atox1 Recombinant antibody

Atox1 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241566A7

Cat no : 84305-1-RR

Synonyms

HAH1, Atx1, 241566A7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Atox1 Recombinant antibody (84305-1-RR)

    WB analysis using 84305-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84305-1-RR (Atox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Atox1 Recombinant antibody (84305-1-RR)

    IHC staining of mouse heart using 84305-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84305-1-RR (Atox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA375 cells, A549 cells, Jurkat cells, mouse kidney tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84305-1-RR is Atox1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Atox1 fusion protein Ag35290 相同性解析による交差性が予測される生物種
Full Name ATX1 (antioxidant protein 1) homolog 1 (yeast)
Calculated molecular weight7 kDa
Observed molecular weight7 kDa
GenBank accession numberNM_009720
Gene symbol Atox1
Gene ID (NCBI) 11927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.

Protocols

Product Specific Protocols
WB protocol for Atox1 antibody 84305-1-RRDownload protocol
IHC protocol for Atox1 antibody 84305-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols