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  • Featured Product
  • KD/KO Validated

B23/NPM1 Monoclonal antibody, PBS Only

B23/NPM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F12A3

Cat no : 60096-1-PBS

Synonyms

B23, B23/NPM1, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    WB analysis of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    WB analysis of ROS1728 using 60096-1-Ig (same clone as 60096-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    WB analysis of NIH/3T3 using 60096-1-Ig (same clone as 60096-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    WB analysis of C6 using 60096-1-Ig (same clone as 60096-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    WB analysis of RAW 264.7 using 60096-1-Ig (same clone as 60096-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    IHC staining of human breast cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    IHC staining of human breast cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    IHC staining of human colon cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    IHC staining of human colon cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    IHC staining of human colon cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    IHC staining of human colon cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    IF Staining of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using B23/NPM1 Monoclonal antibody (60096-1-Ig)

    IF Staining of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60096-1-PBS targets B23/NPM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen B23/NPM1 fusion protein Ag7415 相同性解析による交差性が予測される生物種
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated molecular weight 33 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC002398
Gene symbol NPM1
Gene ID (NCBI) 4869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.