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  • Featured Product
  • KD/KO Validated

B23/NPM1 Recombinant antibody, PBS Only

B23/NPM1 Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4

Cat no : 82030-1-PBS

Synonyms

B23, NPM1, Numatrin, Nucleophosmin, NPM

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using B23/NPM1 Recombinant antibody (82030-1-RR)

    WB analysis of HepG2 using 82030-1-RR (same clone as 82030-1-PBS)

    WB result of B23/NPM1 antibody (82030-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B23/NPM1 transfected HepG2 cells. This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using B23/NPM1 Recombinant antibody (82030-1-RR)

    WB analysis using 82030-1-RR (same clone as 82030-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82030-1-RR (B23 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using B23/NPM1 Recombinant antibody (82030-1-RR)

    IP experiment of Jurkat using 82030-1-RR (same clone as 82030-1-PBS)

    IP result of anti-B23/NPM1 (IP:82030-1-RR, 4ug; Detection:82030-1-RR 1:8000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using B23/NPM1 Recombinant antibody (82030-1-RR)

    IHC staining of human prostate cancer using 82030-1-RR (same clone as 82030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using B23/NPM1 Recombinant antibody (82030-1-RR)

    IHC staining of human prostate cancer using 82030-1-RR (same clone as 82030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human normal colon using B23/NPM1 Recombinant antibody (82030-1-RR)

    IHC staining of human normal colon using 82030-1-RR (same clone as 82030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82030-1-RR (B23/NPM1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using B23/NPM1 Recombinant antibody (82030-1-RR)

    IF Staining of HeLa using 82030-1-RR (same clone as 82030-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using B23 antibody (82030-1-RR, Clone: 1G4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82030-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82030-1-RR

    Affinity and Kinetic Characterization of 82030-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82030-1-RR against Human B23/NPM1 were performed. The affinity constant is 0.409 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82030-1-PBS targets B23/NPM1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen B23/NPM1 fusion protein Ag0286 相同性解析による交差性が予測される生物種
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated molecular weight 33 kDa
Observed molecular weight38 kDa
GenBank accession numberBC002398
Gene symbol NPM1
Gene ID (NCBI) 4869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.