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CoraLite®568-conjugated B23/NPM1 Monoclonal antibody
B23/NPM1 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®568 Fluorescent Dye
CloneNo.
4F12A3
Cat no : CL568-60096
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL568-60096 targets B23/NPM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | B23/NPM1 fusion protein Ag7415 相同性解析による交差性が予測される生物種 |
| Full Name | nucleophosmin (nucleolar phosphoprotein B23, numatrin) |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 35-38 kDa |
| GenBank accession number | BC002398 |
| Gene symbol | NPM1 |
| Gene ID (NCBI) | 4869 |
| RRID | AB_2919713 |
| Conjugate | CoraLite®568 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 568 nm / 587 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL568 B23/NPM1 antibody CL568-60096 | Download protocol |
| FC protocol for CL568 B23/NPM1 antibody CL568-60096 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
