Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
55049-1-AP targets B3GALT6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6 |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | NM_080605 |
| Gene Symbol | B3GALT6 |
| Gene ID (NCBI) | 126792 |
| RRID | AB_10888634 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96L58 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
B3GALT6 belongs to the glycosyltransferase 31 family. It transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. B3GALT6 has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. It has no activity towards substrates with terminal glucosamine or galactosamine residues. The antibody is specific to B3GALT6.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for B3GALT6 antibody 55049-1-AP | Download protocol |
| IHC protocol for B3GALT6 antibody 55049-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Microbiol Sulfated glycosaminoglycans are host epithelial cell targets of the Candida albicans toxin candidalysin | ||
bioRxiv A genome-scale screen identifies sulfated glycosaminoglycans as pivotal in epithelial cell damage by Candida albicans
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