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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B3GAT1 Monoclonal antibody

B3GAT1 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F4A11

Cat no : 66711-1-Ig

Synonyms

GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B3GAT1 Monoclonal antibody (66711-1-Ig)

    WB analysis using 66711-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using B3GAT1 Monoclonal antibody (66711-1-Ig)

    WB analysis of mouse brain using 66711-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of K-562 cells using B3GAT1 Monoclonal antibody (66711-1-Ig)

    FC experiment of K-562 using 66711-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CD57 (66711-1-Ig, Clone:1F4A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inK-562 cells, mouse brain tissue, Jurkat cells, PC-3 cells, SH-SY5Y cells, U-251 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66711-1-Ig targets B3GAT1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen B3GAT1 fusion protein Ag13593 相同性解析による交差性が予測される生物種
Full Name beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa, 100 kDa
GenBank accession numberBC010466
Gene symbol B3GAT1
Gene ID (NCBI) 27087
RRIDAB_2882062
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.

Protocols

Product Specific Protocols
WB protocol for B3GAT1 antibody 66711-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols