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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using B4GALNT1 Polyclonal antibody (13396-1-AP)

    WB analysis of COLO 320 using 13396-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13396-1-AP (B4GALNT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using B4GALNT1 Polyclonal antibody (13396-1-AP)

    WB analysis of A375 using 13396-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 13396-1-AP (B4GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

B4GALNT1 Polyclonal antibody

B4GALNT1 Polyclonal Antibody for WB, ELISA
Cat No. 13396-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

B4GALNT1, GALGT, GalNAc T, GALNACT, GM2/GD2 synthase, SIAT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, A375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13396-1-AP targets B4GALNT1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALNT1 fusion protein Ag4065 相同性解析による交差性が予測される生物種
Full Name beta-1,4-N-acetyl-galactosaminyl transferase 1
Calculated molecular weight 532 aa, 58 kDa
Observed molecular weight 67.5 kDa
GenBank accession numberBC029828
Gene Symbol B4GALNT1
Gene ID (NCBI) 2583
RRIDAB_2061077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00973
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for B4GALNT1 antibody 13396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF,IP

iScience

Linking NMDA Receptor Synaptic Retention to Synaptic Plasticity and Cognition.

Authors - Luca Franchini
View Article
humanWB

Front Genet

Identification of a Novel Glycosyltransferase Prognostic Signature in Hepatocellular Carcinoma Based on LASSO Algorithm.

Authors - Zhiyang Zhou
View Article
humanWB,IHC

J Gene Med

B4GALNT1 promotes carcinogenesis by regulating epithelial-mesenchymal transition in hepatocellular carcinoma based on pan-cancer analysis

Authors - Wenli Liu
  • KD Validated
View Article
humanWB

J Clin Lab Anal

B4GALNT1 Regulates Hepatocellular Carcinoma Cell Proliferation and Apoptosis via the PI3K-AKT-mTOR Pathway

Authors - Lihan Bie
View Article