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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using B4GALNT2 Polyclonal antibody (24024-1-AP)

    WB analysis of Jurkat using 24024-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using B4GALNT2 Polyclonal antibody (24024-1-AP)

    WB analysis of COLO 320 using 24024-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24024-1-AP (B4GALNT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using B4GALNT2 Polyclonal antibody (24024-1-AP)

    IHC staining of human colon using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24024-1-AP (B4GALNT2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using B4GALNT2 Polyclonal antibody (24024-1-AP)

    IHC staining of human colon using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using B4GALNT2 Polyclonal antibody (24024-1-AP)

    IHC staining of human colon using 24024-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24024-1-AP (B4GALNT2 Antibody) at dilution of 1:50 (under 40x lens).

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B4GALNT2 Polyclonal antibody

B4GALNT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24024-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

UDP-GalNAc:Neu5Aca2-3Galb-R b1,4-N-acetylgalactosaminyltransferase, Sd(a) beta-1,4-GalNAc transferase, GALGT2, EC:2.4.1.-, Beta-1,4 N-acetylgalactosaminyltransferase 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24024-1-AP targets B4GALNT2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALNT2 fusion protein Ag21166 相同性解析による交差性が予測される生物種
Full Name beta-1,4-N-acetyl-galactosaminyl transferase 2
Calculated molecular weight 566 aa, 63 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC113677
Gene Symbol B4GALNT2
Gene ID (NCBI) 124872
RRIDAB_2879405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8NHY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B4GALNT2, also named as GALGT2, catalyzes the last step in the biosynthesis of the human Sd(a) antigen, which is found on more than 90% of Caucasian red blood cells. It also catalyzes the last step in the biosynthesis of the Cad antigen, which is related both serologically and biochemically to the Sd(a) antigen. B4GALNT2 transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. This antibody detects the 55-65 kDa B4GALNT2 protein.

Protocols

Product Specific Protocols
WB protocol for B4GALNT2 antibody 24024-1-APDownload protocol
IHC protocol for B4GALNT2 antibody 24024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols