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Validation Data Gallery

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  • Western Blot (WB) analysis of Transfected HEK-293 cells using B4GALNT3 Polyclonal antibody (24675-1-AP)

    WB analysis of Transfected HEK-293 using 24675-1-AP (same clone as 24675-1-PBS)

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 24675-1-AP (B4GALNT3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 24675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using B4GALNT3 Polyclonal antibody (24675-1-AP)

    IHC staining of mouse stomach using 24675-1-AP (same clone as 24675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24675-1-AP (B4GALNT3 antibody) at dilution of 1:200 (under 40x lens).. This data was developed using the same antibody clone with 24675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using B4GALNT3 Polyclonal antibody (24675-1-AP)

    IHC staining of mouse stomach using 24675-1-AP (same clone as 24675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24675-1-AP (B4GALNT3 antibody) at dilution of 1:200 (under 10x lens).. This data was developed using the same antibody clone with 24675-1-PBS in a different storage buffer formulation.

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B4GALNT3 Polyclonal antibody, PBS Only

B4GALNT3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24675-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24675-1-PBS targets B4GALNT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALNT3 fusion protein Ag20342 相同性解析による交差性が予測される生物種
Full Name beta-1,4-N-acetyl-galactosaminyl transferase 3
Calculated molecular weight 998 aa, 115 kDa
Observed molecular weight100 kDa
GenBank accession numberBC156653
Gene Symbol B4GALNT3
Gene ID (NCBI) 283358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6L9W6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

B4GALNT3 (β1,4-N-acetylgalactosaminyltransferase III) has in vitro activity to promote the synthesis of LacdiNAc, an important structure found on glycoproteins expressed by neurons. B4GALNT3 was highly expressed in either differentiated NB or mature ganglion cells (PMID: 21741930). B4GALNT3 is predominantly expressed in the stomach, colon, and testis (PMID: 32267274).