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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B4GALT4 Polyclonal antibody

B4GALT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15200-1-AP

Synonyms

beta4Gal T4, Beta-1,4-GalTase 4, Beta-1,4-galactosyltransferase 4, Beta 1,4 GalTase 4, b4Gal-T4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B4GALT4 Polyclonal antibody (15200-1-AP)

    WB analysis using 15200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15200-1-AP (B4GALT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using B4GALT4 Polyclonal antibody (15200-1-AP)

    WB analysis of mouse skeletal muscle using 15200-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15200-1-AP (B4GALT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using B4GALT4 Polyclonal antibody (15200-1-AP)

    IHC staining of human kidney using 15200-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15200-1-AP (B4GALT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using B4GALT4 Polyclonal antibody (15200-1-AP)

    IHC staining of human kidney using 15200-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15200-1-AP (B4GALT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using B4GALT4 Polyclonal antibody (15200-1-AP)

    IF Staining of A549 using 15200-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using B4GALT4 antibody (15200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Tested Applications

Positive WB detected inCaco-2 cells, mouse skeletal muscle tissue, PC-3 cells, HaCaT cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15200-1-AP targets B4GALT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALT4 fusion protein Ag7348 相同性解析による交差性が予測される生物種
Full Name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
Calculated molecular weight 40 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC004523
Gene symbol B4GALT4
Gene ID (NCBI) 8702
RRIDAB_2290074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for B4GALT4 antibody 15200-1-APDownload protocol
IHC protocol for B4GALT4 antibody 15200-1-APDownload protocol
IF protocol for B4GALT4 antibody 15200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols