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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B4GALT4 Polyclonal antibody

B4GALT4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30478-1-AP

Synonyms

beta4Gal T4, Beta-1,4-GalTase 4, Beta-1,4-galactosyltransferase 4, Beta 1,4 GalTase 4, b4Gal-T4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B4GALT4 Polyclonal antibody (30478-1-AP)

    WB analysis using 30478-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30478-1-AP (B4GALT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using B4GALT4 Polyclonal antibody (30478-1-AP)

    IF Staining of A549 using 30478-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using B4GALT4 antibody (30478-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHT-29 cells, mouse kidney tissue, mouse lung tissue, mouse thymus tissue, rat kidney tissue, rat thymus tissue, mouse cerebellum tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30478-1-AP targets B4GALT4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALT4 fusion protein Ag32337 相同性解析による交差性が予測される生物種
Full Name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
Calculated molecular weight 40 kDa
Observed molecular weight34-40 kDa
GenBank accession numberBC004523
Gene symbol B4GALT4
Gene ID (NCBI) 8702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for B4GALT4 antibody 30478-1-APDownload protocol
IF protocol for B4GALT4 antibody 30478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols