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  • Featured Product
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B4GALT6 Polyclonal antibody

B4GALT6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20148-1-AP

Synonyms

EC:2.4.1.274, EC:2.4.1.-, Beta4Gal-T6, Beta-1,4-GalTase 6, Beta-1,4-galactosyltransferase 6

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using B4GALT6 Polyclonal antibody (20148-1-AP)

    WB analysis of HEK-293 using 20148-1-AP

    WB result of B4GALT6 antibody (20148-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B4GALT6 transfected HEK-293 cells.

  • Western Blot (WB) analysis of mouse heart tissue using B4GALT6 Polyclonal antibody (20148-1-AP)

    WB analysis of mouse heart using 20148-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using B4GALT6 Polyclonal antibody (20148-1-AP)

    WB analysis of mouse kidney using 20148-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using B4GALT6 Polyclonal antibody (20148-1-AP)

    WB analysis of mouse brain using 20148-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using B4GALT6 Polyclonal antibody (20148-1-AP)

    WB analysis of HEK-293 using 20148-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20148-1-AP (B4GALT6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using B4GALT6 Polyclonal antibody (20148-1-AP)

    IHC staining of human colon using 20148-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using B4GALT6 Polyclonal antibody (20148-1-AP)

    IHC staining of human colon using 20148-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20148-1-AP (B4GALT6 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using B4GALT6 Polyclonal antibody (20148-1-AP)

    IHC staining of human brain using 20148-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using B4GALT6 Polyclonal antibody (20148-1-AP)

    IHC staining of human brain using 20148-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20148-1-AP (B4GALT6 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using B4GALT6 Polyclonal antibody (20148-1-AP)

    IF Staining of HeLa using 20148-1-AP

    Immunofluorescent analysis of Hela cells, using B4GALT6 antibody 20148-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, HEK-293 cells, mouse brain tissue
Positive IHC detected inhuman colon tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20148-1-AP targets B4GALT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALT6 fusion protein Ag13686 相同性解析による交差性が予測される生物種
Full Name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
Calculated molecular weight 382 aa, 45 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberBC074884
Gene symbol B4GALT6
Gene ID (NCBI) 9331
RRIDAB_10665357
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-1,4-galactosyltransferase 6 (B4GALT6) is a member of the beta 4-galactosyltransferase family that catalyzes the biosynthesis of glycosphingolipids in UDP-dependent manner (PMID: 23744354). B4GALT6 in astrocytes boosts CNS inflammation and promote neurodegeneration during chronic CNS inflammation (PMID: 25216636).

Protocols

Product Specific Protocols
WB protocol for B4GALT6 antibody 20148-1-APDownload protocol
IHC protocol for B4GALT6 antibody 20148-1-APDownload protocol
IF protocol for B4GALT6 antibody 20148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Med

Regulation of astrocyte activation by glycolipids drives chronic CNS inflammation.

Authors - Lior Mayo
View Article
humanIHC

Biochim Biophys Acta Mol Cell Biol Lipids

Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors.

Authors - Jiřina Procházková
View Article