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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B7-H3/CD276 Polyclonal antibody

B7-H3/CD276 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30052-1-AP

Synonyms

CD276, B7-H3, B7H3, B7 homolog 3, B7 H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B7-H3/CD276 Polyclonal antibody (30052-1-AP)

    WB analysis using 30052-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HuH-7 cells using B7-H3/CD276 Polyclonal antibody (30052-1-AP)

    IP experiment of HuH-7 using 30052-1-AP

    IP result of anti-B7-H3/CD276 (IP:30052-1-AP, 4ug; Detection:30052-1-AP 1:15000) with HuH-7 cells lysate 840 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using B7-H3/CD276 Polyclonal antibody (30052-1-AP)

    IHC staining of human lung cancer using 30052-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using B7-H3/CD276 Polyclonal antibody (30052-1-AP)

    IHC staining of human lung cancer using 30052-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using B7-H3/CD276 Polyclonal antibody (30052-1-AP)

    IHC staining of human skin cancer using 30052-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using B7-H3/CD276 Polyclonal antibody (30052-1-AP)

    IHC staining of human skin cancer using 30052-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30052-1-AP (B7-H3/CD276 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using B7-H3/CD276 Polyclonal antibody (30052-1-AP)

    IF Staining of HEK-293 using 30052-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using B7-H3/CD276 antibody (30052-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, LNCaP cells, U2OS cells
Positive IP detected inHuH-7 cells
Positive IHC detected inhuman lung cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30052-1-AP targets B7-H3/CD276 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD276 molecule
Calculated molecular weight57 kDa
Observed molecular weight95-100 kDa
GenBank accession numberNM_001024736
Gene symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Protocols

Product Specific Protocols
WB protocol for B7-H3/CD276 antibody 30052-1-APDownload protocol
IHC protocol for B7-H3/CD276 antibody 30052-1-APDownload protocol
IF protocol for B7-H3/CD276 antibody 30052-1-APDownload protocol
IP protocol for B7-H3/CD276 antibody 30052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols