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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

    HEK-293, PC-3, LNCaP, HeLa cells, and human spleen, human placenta, pig spleen tissues were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

    WB result of B7-H3/CD276 antibody (66481-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with normal HEK-293 (sh-control ) and three independent sh-B7-H3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    WB analysis of A431 using 66481-1-Ig (same clone as 66481-1-PBS)

    Untreated and PNGase F-treated lysates of A431 cells and PC-3 cells were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:750 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:750 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human prostate cancer using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human prostate cancer using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human lung cancer using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human lung cancer using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IF Staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using B7-H3/CD276 antibody (66481-1-Ig, Clone: 1E7D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

B7-H3 Monoclonal antibody, PBS Only

B7-H3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66481-1-PBS
Clone No.1E7D1

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CD276, B7H3, B7 homolog 3, B7 H3, 4Ig-B7-H3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66481-1-PBS targets B7-H3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen B7-H3 fusion protein Ag6006 相同性解析による交差性が予測される生物種
Full Name CD276 molecule
Calculated molecular weight 57 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC062581
Gene Symbol CD276
Gene ID (NCBI) 80381
RRIDAB_2881847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5ZPR3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).