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  • Featured Product
  • KD/KO Validated

B7-H3 Monoclonal antibody, PBS Only

B7-H3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E7D1

Cat no : 66481-1-PBS

Synonyms

4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

    WB result of B7-H3/CD276 antibody (66481-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with normal HEK-293 (sh-control ) and three independent sh-B7-H3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    WB analysis of HEK-293 using 66481-1-Ig (same clone as 66481-1-PBS)

    HEK-293, PC-3, LNCaP, HeLa cells, and human spleen, human placenta, pig spleen tissues were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    WB analysis of A431 using 66481-1-Ig (same clone as 66481-1-PBS)

    Untreated and PNGase F-treated lysates of A431 cells and PC-3 cells were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human tonsillitis using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3/CD276 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human prostate cancer using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human prostate cancer using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human lung cancer using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human lung cancer using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:750 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IHC staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66481-1-Ig (B7-H3/CD276 antibody at dilution of 1:750 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using B7-H3/CD276 Monoclonal antibody (66481-1-Ig)

    IF Staining of human placenta using 66481-1-Ig (same clone as 66481-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using B7-H3/CD276 antibody (66481-1-Ig, Clone: 1E7D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66481-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66481-1-PBS targets B7-H3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen B7-H3 fusion protein Ag6006 相同性解析による交差性が予測される生物種
Full Name CD276 molecule
Calculated molecular weight 57 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC062581
Gene symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).