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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B7‑H4 Monoclonal antibody, PBS Only

B7‑H4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5B1

Cat no : 66817-1-PBS

Synonyms

VTCN1, B7-H4, B7H4, B7h.5, B7 homolog 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B7‑H4 Monoclonal antibody (66817-1-Ig)

    WB analysis using 66817-1-Ig (same clone as 66817-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66817-1-Ig (B7‑H4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using B7‑H4 Monoclonal antibody (66817-1-Ig)

    IHC staining of human cervical cancer using 66817-1-Ig (same clone as 66817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using B7‑H4 Monoclonal antibody (66817-1-Ig)

    IHC staining of mouse colon using 66817-1-Ig (same clone as 66817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using B7‑H4 Monoclonal antibody (66817-1-Ig)

    IHC staining of rat kidney using 66817-1-Ig (same clone as 66817-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66817-1-PBS targets B7‑H4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen B7‑H4 fusion protein Ag27751 相同性解析による交差性が予測される生物種
Full Name V-set domain containing T cell activation inhibitor 1
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight 31-35 kDa
GenBank accession numberBC065717
Gene symbol B7-H4
Gene ID (NCBI) 79679
RRIDAB_2882160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.