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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B7H6 Polyclonal antibody (27428-1-AP)

    WB analysis using 27428-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27428-1-AP (B7H6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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B7-H6 Polyclonal antibody

B7-H6 Polyclonal Antibody for WB, ELISA
Cat No. 27428-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

B7H6, NCR3LG1, Natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, B7 H6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27428-1-AP targets B7-H6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B7-H6 fusion protein Ag26532 相同性解析による交差性が予測される生物種
Full Name hypothetical protein DKFZp686O24166
Calculated molecular weight51 kDa
Observed molecular weight75-80 kDa
GenBank accession numberBC136800
Gene Symbol B7H6
Gene ID (NCBI) 374383
RRIDAB_3085956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68D85
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B7-H6, also known as NCR3LG1, is a member of the B7 family of immunoreceptors. It is a 454-aa-long type I transmembrane protein consisting of an extracellular region, a transmembrane segment, and a cytoplasmic domain which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), an SH2-binding domain, and an SH3-binding motif. B7-H6 triggers NKp30-mediated activation of human NK cells (PMID: 19528259). It has been reported that B7H6 is expressed in various types of tumor cells and plays a prominent role in natural killer cell immunosurveillance (PMID: 35697295). The apparent molecular weight of B7-H6 is larger than the calculated molecular weight of 51 kDa due to glycosylation (PMID: 19528259).

Protocols

Product Specific Protocols
WB protocol for B7-H6 antibody 27428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols