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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B7H6 Recombinant antibody

B7H6 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240808B2

Cat no : 83908-4-RR

Synonyms

NCR3LG1, Natural cytotoxicity triggering receptor 3 ligand 1, B7 homolog 6, B7 H6, 240808B2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Raji cells using B7H6 Recombinant antibody (83908-4-RR)

    IF Staining of Raji using 83908-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using B7H6 antibody (83908-4-RR, Clone: 240808B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83908-4-RR

    Affinity and Kinetic Characterization of 83908-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83908-4-RR against Human B7H6 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83908-4-RR is B7H6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name hypothetical protein DKFZp686O24166
Calculated molecular weight51 kDa
Observed molecular weight75-80 kDa
GenBank accession numberBC136800
Gene symbol B7H6
Gene ID (NCBI) 374383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B7-H6, also known as NCR3LG1, is a member of the B7 family of immunoreceptors. It is a 454-aa-long type I transmembrane protein consisting of an extracellular region, a transmembrane segment, and a cytoplasmic domain which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), an SH2-binding domain, and an SH3-binding motif. B7-H6 triggers NKp30-mediated activation of human NK cells (PMID: 19528259). It has been reported that B7H6 is expressed in various types of tumor cells and plays a prominent role in natural killer cell immunosurveillance (PMID: 35697295). The apparent molecular weight of B7-H6 is larger than the calculated molecular weight of 51 kDa due to glycosylation (PMID: 19528259).

Protocols

Product Specific Protocols
IF protocol for B7H6 antibody 83908-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols