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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using BACH1 Polyclonal antibody (14018-1-AP)

    WB analysis of HEK-293 using 14018-1-AP (same clone as 14018-1-PBS)

    WB result of BACH1 antibody (14018-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BACH1 transfected HEK-293 cells. This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BACH1 Polyclonal antibody (14018-1-AP)

    WB analysis using 14018-1-AP (same clone as 14018-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14018-1-AP (BACH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using BACH1 Polyclonal antibody (14018-1-AP)

    WB analysis of Raji using 14018-1-AP (same clone as 14018-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 14018-1-AP (BACH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using BACH1 Polyclonal antibody (14018-1-AP)

    WB analysis of MCF-7 using 14018-1-AP (same clone as 14018-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14018-1-AP (BACH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using BACH1 Polyclonal antibody (14018-1-AP)

    IHC staining of human cervical cancer using 14018-1-AP (same clone as 14018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using BACH1 Polyclonal antibody (14018-1-AP)

    IHC staining of human breast hyperplasia using 14018-1-AP (same clone as 14018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using BACH1 Polyclonal antibody (14018-1-AP)

    IHC staining of human breast hyperplasia using 14018-1-AP (same clone as 14018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using BACH1 Polyclonal antibody (14018-1-AP)

    IHC staining of human cervical cancer using 14018-1-AP (same clone as 14018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BACH1 Polyclonal antibody (14018-1-AP)

    IHC staining of human breast cancer using 14018-1-AP (same clone as 14018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BACH1 Polyclonal antibody (14018-1-AP)

    IHC staining of human breast cancer using 14018-1-AP (same clone as 14018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14018-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

BACH1 Polyclonal antibody, PBS Only

BACH1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14018-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14018-1-PBS targets BACH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BACH1 fusion protein Ag5128 相同性解析による交差性が予測される生物種
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated molecular weight 82 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC063307
Gene Symbol BACH1
Gene ID (NCBI) 571
ENSEMBL Gene IDENSG00000156273
RRIDAB_2274498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14867
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BTB and CNC homology 1 (Bach1) is a transcription factor expressed in many tissues, with key roles in cell cycle 

regulation in homeostasis and in cancer in disease.


What is the molecular weight of BACH1?

Bach1 consists of 736 amino acids and is a member of the cap'n'collar type of basic region leucine zipper factor 

family (CNC-bZip) with several atypical protein domains. Although its predicted MW is 82 kDa, it runs 

anomalously at 100-110 kDa in SDS-PAGE.


What is the cellular localization of BACH1?

As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms a 

heterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to the 

Maf recognition elements (MAREs) of target genes. During oxidative stress, BACH1 can be inactivated either by 

heme or by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes to 

microtubules (PMID: 15809329).


What is the function of BACH1?

BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as heme 

oxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involved 

in the oxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced by nuclear factor 

(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays a 

role in cell proliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6 

(CDK6) and BCL2-like 11 (BCL2L11).


What is the role of BACH1 in disease?

BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The function 

of BACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis, 

influence epigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787

892). BACH1 may act on the same gene in different contexts to repress or activate it (PMID: 8887638).