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  • Featured Product
  • KD/KO Validated

BACH1 Monoclonal antibody, PBS Only (Capture)

BACH1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D12A4

Cat no : 66762-1-PBS

Synonyms

BACH-1, BACH 1, 2D12A4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BACH1 Monoclonal antibody (66762-1-Ig)

    WB analysis using 66762-1-Ig (same clone as 66762-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66762-1-Ig (BACH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using BACH1 Monoclonal antibody (66762-1-Ig)

    WB analysis of HEK-293 using 66762-1-Ig (same clone as 66762-1-PBS)

    WB result of BACH1 antibody (66762-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BACH1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using BACH1 Monoclonal antibody (66762-1-Ig)

    WB analysis of HSC-T6 using 66762-1-Ig (same clone as 66762-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66762-1-Ig (BACH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BACH1 Monoclonal antibody (66762-1-Ig)

    IHC staining of human lung cancer using 66762-1-Ig (same clone as 66762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BACH1 Monoclonal antibody (66762-1-Ig)

    IHC staining of human lung cancer using 66762-1-Ig (same clone as 66762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP80007-3

    Cytometric bead array standard curve of MP80007-3

    Cytometric bead array standard curve of MP80007-3, BACH1 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 66762-1-PBS. Detection antibody: 82842-2-PBS. Standard: Ag6011. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP50384-1

    Cytometric bead array standard curve of MP50384-1

    Cytometric bead array standard curve of MP50384-1, BACH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66762-1-PBS. Detection antibody: 66762-2-PBS. Standard:Ag6011. Range: 6.25-200 ng/mL.

  • Cytometric bead array standard curve of MP50384-2

    Cytometric bead array standard curve of MP50384-2

    Cytometric bead array standard curve of MP50384-2, BACH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66762-1-PBS. Detection antibody: 66762-3-PBS. Standard:Ag6011. Range: 6.25-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66762-1-PBS targets BACH1 as part of a matched antibody pair:

MP50384-1: 66762-1-PBS capture and 66762-2-PBS detection (validated in Cytometric bead array)

MP50384-2: 66762-1-PBS capture and 66762-3-PBS detection (validated in Cytometric bead array)

MP80007-3: 66762-1-PBS capture and 82842-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BACH1 fusion protein Ag6011 相同性解析による交差性が予測される生物種
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated molecular weight 82 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC063307
Gene symbol BACH1
Gene ID (NCBI) 571
RRIDAB_2882108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.