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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BACH1 Recombinant antibody (82842-7-RR)

    WB analysis using 82842-7-RR (same clone as 82842-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82842-7-RR (BACH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82842-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BACH1 Recombinant antibody (82842-7-RR)

    IF Staining of HeLa using 82842-7-RR (same clone as 82842-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BACH1 antibody (82842-7-RR, Clone: 4K14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82842-7-PBS in a different storage buffer formulation.

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BACH1 Recombinant antibody, PBS Only

BACH1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82842-7-PBS
Clone No.4K14

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BACH-1, BACH 1, 4K14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82842-7-PBS targets BACH1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BACH1 fusion protein Ag5128 相同性解析による交差性が予測される生物種
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated molecular weight 82 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC063307
Gene Symbol BACH1
Gene ID (NCBI) 571
ENSEMBL Gene IDENSG00000156273
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14867
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.