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Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using BACH2 Polyclonal antibody (27635-1-AP)

    WB analysis of Daudi using 27635-1-AP (same clone as 27635-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27635-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using BACH2 Polyclonal antibody (27635-1-AP)

    WB analysis of Ramos using 27635-1-AP (same clone as 27635-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27635-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using BACH2 Polyclonal antibody (27635-1-AP)

    WB analysis of Daudi using 27635-1-AP (same clone as 27635-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27635-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27635-1-PBS targets BACH2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BACH2 fusion protein Ag26526 相同性解析による交差性が予測される生物種
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 2
Calculated molecular weight 93 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberNM_001170794
Gene Symbol BACH2
Gene ID (NCBI) 60468
RRIDAB_2880934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BACH2, also named as Transcription regulator protein BACH2, is a 841 amino acid protein, which is widely expressed within the B-lymphoid lineage, except in plasma cells. BACH2 contains an N-terminal BTB domain involved in its transcriptional function, but instead of having zinc fingers at the C-terminus, it binds to DNA through a basic leucine zipper motif. BACH2 binds DNA consensus sequences, termed MARE motifs, by forming a heterodimer with small leucine zipper MAF proteins such as MAFK, MAFG, and MAFF. The calculated molecular weight of BACH2 is 92 kDa, but the post-modifiction of BACH2 is about 120-130 kDa. (PMID: 24277074 )