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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using BAF53B Polyclonal antibody (27215-1-AP)

    WB analysis of mouse brain using 27215-1-AP (same clone as 27215-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27215-1-AP (BAF53B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using BAF53B Polyclonal antibody (27215-1-AP)

    WB analysis of rat brain using 27215-1-AP (same clone as 27215-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 27215-1-AP (BAF53B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27215-1-PBS in a different storage buffer formulation.

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BAF53B Polyclonal antibody, PBS Only

BAF53B Polyclonal Antibody for WB, Indirect ELISA
Cat No. 27215-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27215-1-PBS targets BAF53B in WB, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAF53B fusion protein Ag25745 相同性解析による交差性が予測される生物種
Full Name actin-like 6B
Calculated molecular weight 47 kDa
Observed molecular weight 47-53 kDa
GenBank accession numberBC020944
Gene Symbol BAF53B
Gene ID (NCBI) 51412
RRIDAB_2857952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94805
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BAF53B, also named as ACTL6B, encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. BAF53b is unique among nucleosome remodeling complex subunits because it is neuron specific and is not found in any other nucleosome remodeling complex besides the nBAF complex(PMID: 23525042). BAF53B can be detected as about 47-53 kDa.