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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAFF Recombinant antibody (84541-3-RR)

    WB analysis using 84541-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84541-3-RR (BAFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using BAFF Recombinant antibody (84541-3-RR)

    WB analysis of U-937 using 84541-3-RR

    U-937 cells were subjected to SDS PAGE followed by western blot with 84541-3-RR (BAFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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BAFF Recombinant antibody

BAFF Recombinant Antibody for WB, ELISA
Cat No. 84541-3-RR
Clone No.242006E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

242006E4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-937 cells, Jurkat cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84541-3-RR targets BAFF in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 13b
Calculated molecular weight34kd
Observed molecular weight 32kd, 17kd
GenBank accession numberNM_033622
Gene Symbol Tnfsf13b
Gene ID (NCBI) 24099
RRIDAB_3672046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9WU72
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAFF (B-cell-activating factor), also called Tnfsf13b (Tumor necrosis factor ligand superfamily member 13B) and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in stimulating B- and T-cell function and the regulation of humoral immunity. A third B-cell-specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response (PMID: 12867412). BAFF cleaved into 2 chains: membrane form is 32-35 kDa; soluble form is 17 kDa.

Protocols

Product Specific Protocols
WB protocol for BAFF antibody 84541-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols