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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    WB result of BAG1 antibody (19064-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG1 transfected HeLa cells. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of UV treated Hela using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of UV treated Hela using 19064-1-AP (same clone as 19064-1-PBS)

    UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of Jurkat using 19064-1-AP (same clone as 19064-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of MCF-7 using 19064-1-AP (same clone as 19064-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HEK-293 using 19064-1-AP (same clone as 19064-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW264.7 using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of RAW264.7 using 19064-1-AP (same clone as 19064-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HT-1080 using 19064-1-AP (same clone as 19064-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of HL-60 using 19064-1-AP (same clone as 19064-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using BAG1 Polyclonal antibody (19064-1-AP)

    WB analysis of MCF-7 using 19064-1-AP (same clone as 19064-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 19064-1-AP (BAG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    IP experiment of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    IP result of anti-BAG1 (IP:19064-1-AP, 3ug; Detection:19064-1-AP 1:500) with HeLa cells lysate 3440ug. This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human testis using 19064-1-AP (same clone as 19064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human testis using 19064-1-AP (same clone as 19064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human ovary using 19064-1-AP (same clone as 19064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using BAG1 Polyclonal antibody (19064-1-AP)

    IHC staining of human ovary using 19064-1-AP (same clone as 19064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 19064-1-AP (BAG1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAG1 Polyclonal antibody (19064-1-AP)

    IF Staining of HeLa using 19064-1-AP (same clone as 19064-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAG1 antibody (19064-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BAG1 Polyclonal antibody (19064-1-AP)

    IF Staining of MCF-7 using 19064-1-AP (same clone as 19064-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using BAG1 antibody 19064-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19064-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

BAG1 Polyclonal antibody, PBS Only

BAG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19064-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19064-1-PBS targets BAG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAG1 fusion protein Ag13555 相同性解析による交差性が予測される生物種
Full Name BCL2-associated athanogene
Calculated molecular weight 39 kDa
Observed molecular weight 50-55 kDa, 30-33 kDa
GenBank accession numberBC014774
Gene Symbol BAG1
Gene ID (NCBI) 573
RRIDAB_10640574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99933
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.