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  • Featured Product
  • KD/KO Validated

BAG1S/1M/1L Polyclonal antibody

BAG1S/1M/1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16150-1-AP

Synonyms

BAG1, BAG1S/1M/1L

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    WB analysis of mouse brain using 16150-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    WB analysis of Jurkat using 16150-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    WB analysis of mouse liver using 16150-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    WB analysis of mouse brain using 16150-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human testis using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human testis using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human spleen using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human spleen using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human lung using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human lung using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human ovary using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human ovary using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human kidney using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human kidney using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human placenta using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using BAG1S/1M/1L Polyclonal antibody (16150-1-AP)

    IHC staining of human placenta using 16150-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16150-1-AP (BAG1S/1M/1L antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, Jurkat cells, mouse liver tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16150-1-AP targets BAG1S/1M/1L in WB, IHC, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name BCL2-associated athanogene
Calculated molecular weight 26 kDa
Observed molecular weight 46-50 kDa, 41-46 kDa, 25-33 kDa
GenBank accession numberNM_004323
Gene symbol BAG1
Gene ID (NCBI) 573
RRIDAB_2062288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAG1 has been identified that modulates gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M, and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody recognizes three isoforms of BAG1, includes GAG1L, BAG1M, and BAG1S.

Protocols

Product Specific Protocols
WB protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
IHC protocol for BAG1S/1M/1L antibody 16150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Tumour Biol

Silencing Bag-1 gene via magnetic gold nanoparticle-delivered siRNA plasmid for colorectal cancer therapy in vivo and in vitro.

Authors - Wenbai Huang
  • KD Validated
View Article