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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAG3 Monoclonal antibody (68076-1-Ig)

    WB analysis using 68076-1-Ig (same clone as 68076-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68076-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BAG3 Monoclonal antibody (68076-1-Ig)

    WB analysis using 68076-1-Ig (same clone as 68076-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68076-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BAG3 Monoclonal antibody (68076-1-Ig)

    WB analysis using 68076-1-Ig (same clone as 68076-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68076-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BAG3 Monoclonal antibody (68076-1-Ig)

    IF Staining of HepG2 using 68076-1-Ig (same clone as 68076-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using BAG3 antibody (68076-1-Ig, Clone: 4G6C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68076-1-PBS in a different storage buffer formulation.

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BAG3 Monoclonal antibody, PBS Only

BAG3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68076-1-PBS
Clone No.4G6C2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

Docking protein CAIR-1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3, BAG-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68076-1-PBS targets BAG3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BAG3 fusion protein Ag17608 相同性解析による交差性が予測される生物種
Full Name BCL2-associated athanogene 3
Calculated molecular weight 61 kDa
Observed molecular weight74 kDa
GenBank accession numberBC006418
Gene Symbol BAG3
Gene ID (NCBI) 9531
RRIDAB_2918814
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95817
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy.