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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SODD/BAG4 Polyclonal antibody

SODD/BAG4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13913-1-AP

Synonyms

SODD, BAG4, BAG 4, Silencer of death domains, Bcl-2-associated athanogene 4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using SODD/BAG4 Polyclonal antibody (13913-1-AP)

    WB analysis of K-562 using 13913-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SODD/BAG4 Polyclonal antibody (13913-1-AP)

    WB analysis of MCF-7 using 13913-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using SODD/BAG4 Polyclonal antibody (13913-1-AP)

    IP experiment of K-562 using 13913-1-AP

    IP result of anti-SODD/BAG4 (IP:13913-1-AP, 4ug; Detection:13913-1-AP 1:500) with K-562 cells lysate 2800ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SODD/BAG4 Polyclonal antibody (13913-1-AP)

    IF Staining of MCF-7 using 13913-1-AP

    Immunofluorescent analysis of MCF-7 cells using 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13913-1-AP targets SODD/BAG4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityartemia sinica
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SODD/BAG4 fusion protein Ag4905 相同性解析による交差性が予測される生物種
Full Name BCL2-associated athanogene 4
Calculated molecular weight 457 aa, 50 kDa
Observed molecular weight 60 kDa, 66-70 kDa
GenBank accession numberBC038505
Gene symbol SODD/BAG4
Gene ID (NCBI) 9530
RRIDAB_2243308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).

Protocols

Product Specific Protocols
WB protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
IF protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
IP protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
Artemia sinicaWB

Gene

Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.

Authors - Mengchen Zhang
View Article
WB

Nat Commun

Mettl3-dependent m6A modification attenuates the brain stress response in Drosophila

Authors - Alexandra E. Perlegos
View Article