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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IRSp53 Monoclonal antibody

IRSp53 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D4G1

Cat no : 68348-1-Ig

Synonyms

BAI associated protein 2, BAI1 associated protein 2, BAIAP2, BAP2, Fas ligand associated factor 3, FLAF3, Insulin receptor substrate p53, IRS 58, IRSP53, IRSp53/58, Protein BAP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IRSp53 Monoclonal antibody (68348-1-Ig)

    WB analysis using 68348-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using IRSp53 Monoclonal antibody (68348-1-Ig)

    WB analysis of LNCaP using 68348-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit cerebellum tissue using IRSp53 Monoclonal antibody (68348-1-Ig)

    WB analysis of rabbit cerebellum using 68348-1-Ig

    rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inrabbit brain tissue, LNCaP cells, rabbit cerebellum tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68348-1-Ig targets IRSp53 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IRSp53 fusion protein Ag25161 相同性解析による交差性が予測される生物種
Full Name BAI1-associated protein 2
Calculated molecular weight 61 kDa
Observed molecular weight53 kDa
GenBank accession numberBC014020
Gene symbol IRSp53
Gene ID (NCBI) 10458
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).

Protocols

Product Specific Protocols
WB protocol for IRSp53 antibody 68348-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols