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Species Reactivity

Species Reactivity

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Conjugate

BAIAP2L1 Polyclonal antibody

BAIAP2L1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25692-1-AP

Synonyms

BAIAP2L1, IRTKS

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of HeLa using 25692-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of K-562 using 25692-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of SGC-7901 using 25692-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human ovary tumor using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human ovary tumor using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human stomach cancer using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human stomach cancer using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, SGC-7901 cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25692-1-AP is BAIAP2L1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAIAP2L1 fusion protein Ag22499 相同性解析による交差性が予測される生物種
Full Name BAI1-associated protein 2-like 1
Calculated molecular weight 511 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC013888
Gene symbol BAIAP2L1
Gene ID (NCBI) 55971
RRIDAB_2880197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAIAP2L1, also known as IRTKS, is a 511-amino acid protein containing an IRSp53/MIM homology domain (IMD) that bundles actin filaments and interacts with the small GTPase Rac (PMID: 17430976; 14752106). In addition to the IMD domain, BAIAP2L1 also contains an SH3 domain and WW domain interaction motif. BAIAP2L1 is widely distributed and is an INS receptor substrate. It has a role in actin remodeling and membrane protrusion.

Protocols

Product Specific Protocols
WB protocol for BAIAP2L1 antibody 25692-1-APDownload protocol
IHC protocol for BAIAP2L1 antibody 25692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Med Oncol

IRTKS contributes to the malignant progression of cervical cancer cells

Authors - Yan Zhang
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects both human and mouse versions of the protein.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T and overexpression
BAIAP2L1 Antibody Western Blot, validation (1:1000 dilution) in 293T and overexpression (Cat no:25692-1-AP)