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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAK Polyclonal antibody (30017-1-AP)

    WB analysis using 30017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BAK Polyclonal antibody (30017-1-AP)

    WB analysis using 30017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using BAK Polyclonal antibody (30017-1-AP)

    IP experiment of HEK-293 using 30017-1-AP

    IP result of anti-BAK (IP:30017-1-AP, 4ug; Detection:30017-1-AP 1:6000) with HEK-293 cells lysate 1640 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAK Polyclonal antibody (30017-1-AP)

    IF Staining of HeLa using 30017-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BAK antibody (30017-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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BAK Polyclonal antibody

BAK Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 30017-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

BAK1, CDN1, Bcl-2-like protein 7, Bcl2-L-7, Bcl-2 homologous antagonist/killer

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Neuro-2a cells, PC-12 cells, HeLa cells, THP-1 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30017-1-AP targets BAK in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityduck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAK fusion protein Ag27958 相同性解析による交差性が予測される生物種
Full Name BCL2-antagonist/killer 1
Calculated molecular weight 23 kDa
Observed molecular weight23-25 kDa
GenBank accession numberBC004431
Gene Symbol BAK1
Gene ID (NCBI) 578
RRIDAB_3086211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16611
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Protocols

Product Specific Protocols
WB protocol for BAK antibody 30017-1-APDownload protocol
IF protocol for BAK antibody 30017-1-APDownload protocol
IP protocol for BAK antibody 30017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
duckWB

J Virol

Duck Tembusu virus NS3 protein induces apoptosis by activating the PERK/PKR pathway and mitochondrial pathway

Authors - Yuhong Pan
View Article