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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BAK Polyclonal antibody

BAK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30017-1-AP

Synonyms

Apoptosis regulator BAK, BAK, BAK LIKE, BAK1, CDN1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using BAK Polyclonal antibody (30017-1-AP)

    WB analysis using 30017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BAK Polyclonal antibody (30017-1-AP)

    WB analysis using 30017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30017-1-AP (BAK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, Neuro-2a cells, PC-12 cells, HeLa cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30017-1-AP targets BAK in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityduck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAK fusion protein Ag27958 相同性解析による交差性が予測される生物種
Full Name BCL2-antagonist/killer 1
Calculated molecular weight 23 kDa
Observed molecular weight23 kDa
GenBank accession numberBC004431
Gene symbol BAK1
Gene ID (NCBI) 578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Protocols

Product Specific Protocols
WB protocol for BAK antibody 30017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
duckWB

J Virol

Duck Tembusu virus NS3 protein induces apoptosis by activating the PERK/PKR pathway and mitochondrial pathway

Authors - Yuhong Pan
View Article