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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BARHL2 Polyclonal antibody

BARHL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23976-1-AP

Synonyms

BarH like 2 homeobox protein, BarH like homeobox 2, BARHL2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using BARHL2 Polyclonal antibody (23976-1-AP)

    WB analysis of HepG2 using 23976-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23976-1-AP (BARHL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using BARHL2 Polyclonal antibody (23976-1-AP)

    WB analysis of NIH/3T3 using 23976-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23976-1-AP (BARHL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using BARHL2 Polyclonal antibody (23976-1-AP)

    IHC staining of human testis using 23976-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23976-1-AP (BARHL2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using BARHL2 Polyclonal antibody (23976-1-AP)

    IHC staining of human testis using 23976-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23976-1-AP (BARHL2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BARHL2 Polyclonal antibody (23976-1-AP)

    IF Staining of HepG2 using 23976-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (23976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23976-1-AP targets BARHL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BARHL2 fusion protein Ag21156 相同性解析による交差性が予測される生物種
Full Name BarH-like homeobox 2
Calculated molecular weight 387 aa, 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC126439
Gene symbol BARHL2
Gene ID (NCBI) 343472
RRIDAB_2879386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.

Protocols

Product Specific Protocols
WB protocol for BARHL2 antibody 23976-1-APDownload protocol
IHC protocol for BARHL2 antibody 23976-1-APDownload protocol
IF protocol for BARHL2 antibody 23976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Aging

Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging

Authors - Meiyang Li
View Article