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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BAT1 Monoclonal antibody, PBS Only

BAT1 Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G9H8

Cat no : 67641-1-PBS

Synonyms

DDX39B, UAP56, DDX39, D6S81E, ATP-dependent RNA helicase p47

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAT1 Monoclonal antibody (67641-1-Ig)

    WB analysis using 67641-1-Ig (same clone as 67641-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67641-1-Ig (BAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67641-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using BAT1 Monoclonal antibody (67641-1-Ig)

    IP experiment of HeLa using 67641-1-Ig (same clone as 67641-1-PBS)

    IP result of anti-BAT1 (IP:67641-1-Ig, 4ug; Detection:67641-1-Ig 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 67641-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BAT1 Monoclonal antibody (67641-1-Ig)

    IHC staining of human breast cancer using 67641-1-Ig (same clone as 67641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67641-1-Ig (BAT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67641-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BAT1 Monoclonal antibody (67641-1-Ig)

    IHC staining of human breast cancer using 67641-1-Ig (same clone as 67641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67641-1-Ig (BAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67641-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67641-1-PBS targets BAT1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BAT1 fusion protein Ag6612 相同性解析による交差性が予測される生物種
Full Name HLA-B associated transcript 1
Calculated molecular weight 53 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC000361
Gene symbol BAT1
Gene ID (NCBI) 7919
RRIDAB_2882841
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.