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  • Featured Product
  • KD/KO Validated

BAX Monoclonal antibody, PBS Only

BAX Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G5E8

Cat no : 60267-1-PBS

Synonyms

Bcl-2-like protein 4, Bcl2-L-4, Apoptosis regulator BAX, 4G5E8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAX Monoclonal antibody (60267-1-Ig)

    WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BAX Monoclonal antibody (60267-1-Ig)

    WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

    EC109 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using BAX Monoclonal antibody (60267-1-Ig)

    WB analysis of human testis using 60267-1-Ig (same clone as 60267-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using BAX Monoclonal antibody (60267-1-Ig)

    WB analysis of HepG2 using 60267-1-Ig (same clone as 60267-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of THP-1 cells using BAX Monoclonal antibody (60267-1-Ig)

    IP experiment of THP-1 using 60267-1-Ig (same clone as 60267-1-PBS)

    IP result of anti-BAX (IP:60267-1-Ig, 4ug; Detection:60267-1-Ig 1:6000) with THP-1 cells lysate 1520 ug. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BAX Monoclonal antibody (60267-1-Ig)

    IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BAX Monoclonal antibody (60267-1-Ig)

    IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BAX Monoclonal antibody (60267-1-Ig)

    IF Staining of MCF-7 using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using BAX antibody (60267-1-Ig, Clone: 4G5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60267-1-PBS targets BAX in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BAX fusion protein Ag21068 相同性解析による交差性が予測される生物種
Full Name BCL2-associated X protein
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC014175
Gene symbol BAX
Gene ID (NCBI) 581
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).