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Validation Data Gallery

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  • Western Blot (WB) analysis of TT cells using BBOF1 Polyclonal antibody (24337-1-AP)

    WB analysis of TT using 24337-1-AP

    TT cells were subjected to SDS PAGE followed by western blot with 24337-1-AP (CCDC176 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BBOF1 Polyclonal antibody (24337-1-AP)

    WB analysis using 24337-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24337-1-AP (BBOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BBOF1 Polyclonal antibody (24337-1-AP)

    IHC staining of mouse testis using 24337-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BBOF1 Polyclonal antibody (24337-1-AP)

    IHC staining of mouse testis using 24337-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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BBOF1 Polyclonal antibody

BBOF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24337-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C14orf45, Coiled-coil domain-containing protein 176, CCDC176, Basal body-orientation factor 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, TT cells, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24337-1-AP targets BBOF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBOF1 fusion protein Ag19302 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 45
Calculated molecular weight 529 aa, 62 kDa
Observed molecular weight62 kDa
GenBank accession numberBC104981
Gene Symbol BBOF1
Gene ID (NCBI) 80127
RRIDAB_3669433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8ND07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Basal body orientation factor 1 (BBOF1), also known as CCDC176, contains two coiled-coil domains and is evolutionarily conserved in vertebrates. Recently, Xenopus bbof1 has been characterized as a cilia-associated factor that is induced by the transcription factor foxj1 to mediate the alignment and orientation of cilia in epidermal multi-ciliated cells. BBOF1 is required for sperm flagellar axoneme maintenance and male fertility in mice. BBOF1 expression is elevated during meiosis, and it is accumulated in round spermatids. BBOF1 deletion has compromised effects on growth, survival, and spermatogenesis.

Protocols

Product Specific Protocols
WB protocol for BBOF1 antibody 24337-1-APDownload protocol
IHC protocol for BBOF1 antibody 24337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols