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Species Reactivity

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  • Featured Product
  • KD/KO Validated

BBOX1 Polyclonal antibody

BBOX1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16099-1-AP

Synonyms

Gamma-BBH, gamma BBH, G BBH, EC:1.14.11.1, BBOX

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using BBOX1 Polyclonal antibody (16099-1-AP)

    WB analysis of human brain using 16099-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16099-1-AP (BBOX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using BBOX1 Polyclonal antibody (16099-1-AP)

    WB analysis of human brain using 16099-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16099-1-AP (BBOX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BBOX1 Polyclonal antibody (16099-1-AP)

    IF Staining of HepG2 using 16099-1-AP

    Immunofluorescent analysis of HepG2 cells, using BBOX1 antibody 16099-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16099-1-AP targets BBOX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBOX1 fusion protein Ag8964 相同性解析による交差性が予測される生物種
Full Name butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1
Calculated molecular weight 387 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC011034
Gene symbol BBOX1
Gene ID (NCBI) 8424
RRIDAB_2243498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BBOX1 antibody 16099-1-APDownload protocol
IF protocol for BBOX1 antibody 16099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

EMBO J

CRIP1 suppresses BBOX1-mediated carnitine metabolism to promote stemness in hepatocellular carcinoma

Authors - Jing Wang
View Article
humanWB,IHC

Biochim Biophys Acta Mol Cell Res

BBOX1 mediates metabolic reprogramming driven by hypoxia and participates in the malignant progress of high-grade serous ovarian cancer

Authors - Jiazhen Huang
  • KD Validated
View Article